NASA is expected to give details on the discovery during a news conference on Friday.
The small science probe landed safely last month on a frozen desert at the Martian north pole to search for water and assess conditions for sustaining life.
Small chunks of bright material described as the size of dice have disappeared from inside a trench where they were photographed by the craft earlier this week, NASA said in a statement late on Thursday.
This has convinced scientists the chunks were ice -- frozen water -- that vaporized after digging exposed it, NASA said.
"It must be ice," said mission principal investigator Peter Smith of the University of Arizona. "These little clumps completely disappearing over the course of a few days. That is perfect evidence that it's ice. There had been some question whether the bright material was salt. Salt can't do that."
The presence of water on Mars is a hot topic for scientists. They have presented strong evidence in recent years of huge deposits of frozen water at the Martian poles and point to geological features that indicate that large bodies of water have flowed on the planet's surface in the distant past.
Water is a key to the question of whether life, even in the form of mere microbes, has ever existed on Mars. On Earth, water is a necessary ingredient for life.
The chunks were left at the bottom of a trench dubbed "Dodo-Goldilocks" when Phoenix's robotic arm enlarged that trench on June 15. Several chunks were gone when Phoenix looked at the trench again on Thursday, NASA said.
The U.S. space agency also said that the lander, digging in a different trench, used its robotic arm to connect with a hard surface that has scientists believing they have found an icy layer on the Martian surface.
The $420 million lander spent 10 months journeying from Earth to Mars.
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